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biotinylated α flag  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc biotinylated α flag
    Biotinylated α Flag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+flag+antibodies/DYKDDDDK+Tag+Antibody/pmc11394063-136-7-9
    Average 93 stars, based on 110 article reviews
    biotinylated α flag - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Degradation Assay:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Control:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Microarray:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    In Vitro:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Labeling:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Incubation:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Ubiquitin Proteomics:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    SDS Page:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Autoradiography:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Variant Assay:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Mutagenesis:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Comparison:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Expressing:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Diffusion-based Assay:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Negative Control:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Immunofluorescence:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.

    Fluorescence:

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated antiGFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 µg/µl biotinylated anti-Flag antibodies (Cell Signaling; #2908 S Danvers, MA, USA) were applied.. The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.The antibodies bound to the exposed NutraAvidin, specifically to the area under the ‘button’, creating an array of anti-GFP - or anti-Flag tag.

    Article Title: Protein degradation analysis by affinity microfluidics
    Article Snippet: The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.The ‘button’ valve is then closed, and biotinylated-PEG (1 μg/μl, (PG2-AMBN-5k, Nanocs Inc.) is flowed over for 20 min, passivating the flow layer, except for the buttons area.. Following passivation, the ‘button’ valve is released and a flow of 0.2 μg/μl biotinylated anti-GFP antibodies (Abcam; #ab6658, Cambridge, United Kingdom) or 0.01 μg/μl biotinylated anti-Flag antibodies (Cell Signaling; #2908S Danvers, MA, USA) were applied.. The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.The antibodies bound to the exposed Neutravidin, specifically to the area under the ‘button’, creating an array of anti-GFP – or anti-Flag tag.



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    Millipore biotinylated mouse anti-flag monoclonal m2 antibody
    8-aza-7-deazaguanine (7dzG) substitution reduces BG4 affinity for telomeric constructs. ( A ) Chemical structure of 7dzG in a G-quartet demonstrating loss of Hoogsteen bonding with adjacent guanine. ( B ) Schematic of <t>5′biotinylated</t> DNA constructs containing an 18-base pair stem. Complete sequences shown in . ( C ) ELISA binding curves for 7-dzG-modified telomeric constructs. BG4 binding is represented by absorbance at 450 nm versus the BG4 concentration. ( D ) Apparent dissociation constants ( K d ) were calculated from the nonlinear regression curves shown in ( C ). Data are mean ± SD from 3–5 independent experiments; ordinary one-way ANOVA; * P < 0.1, **** P < 0.0001.
    Biotinylated Mouse Anti Flag Monoclonal M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    8-aza-7-deazaguanine (7dzG) substitution reduces BG4 affinity for telomeric constructs. ( A ) Chemical structure of 7dzG in a G-quartet demonstrating loss of Hoogsteen bonding with adjacent guanine. ( B ) Schematic of 5′biotinylated DNA constructs containing an 18-base pair stem. Complete sequences shown in . ( C ) ELISA binding curves for 7-dzG-modified telomeric constructs. BG4 binding is represented by absorbance at 450 nm versus the BG4 concentration. ( D ) Apparent dissociation constants ( K d ) were calculated from the nonlinear regression curves shown in ( C ). Data are mean ± SD from 3–5 independent experiments; ordinary one-way ANOVA; * P < 0.1, **** P < 0.0001.

    Journal: Nucleic Acids Research

    Article Title: BG4 antibody can recognize telomeric G-quadruplexes harboring destabilizing base modifications and lesions

    doi: 10.1093/nar/gkad1209

    Figure Lengend Snippet: 8-aza-7-deazaguanine (7dzG) substitution reduces BG4 affinity for telomeric constructs. ( A ) Chemical structure of 7dzG in a G-quartet demonstrating loss of Hoogsteen bonding with adjacent guanine. ( B ) Schematic of 5′biotinylated DNA constructs containing an 18-base pair stem. Complete sequences shown in . ( C ) ELISA binding curves for 7-dzG-modified telomeric constructs. BG4 binding is represented by absorbance at 450 nm versus the BG4 concentration. ( D ) Apparent dissociation constants ( K d ) were calculated from the nonlinear regression curves shown in ( C ). Data are mean ± SD from 3–5 independent experiments; ordinary one-way ANOVA; * P < 0.1, **** P < 0.0001.

    Article Snippet: Initially, the biotinylated mouse anti-FLAG monoclonal M2 antibody (F9291, Sigma) was diluted ∼500 times in T50 buffer (10 mM Tris–HCl, pH 7.5, 50 mM NaCl) and immobilized in the imaging chamber via biotin−NeutrAvidin interaction.

    Techniques: Construct, Enzyme-linked Immunosorbent Assay, Binding Assay, Modification, Concentration Assay

    BG4 addition promotes GQ folding of telomeric constructs containing a destabilizing G substitution. Schematics for biotinylated FRET constructs TELO4 ( A ), G2T ( B ), or 8oxoG2 ( C ) tethered to neutravidin coated slides, followed by 150 nM BG4 addition and then buffer washes. Construct sequences shown in . Histograms of FRET efficiency frequencies observed TELO4 ( D ), G2T ( E ), and 8oxoG2 ( F ) before or after BG4 addition, and sequential buffer wash and SDS addition as indicated. Representative single-molecule traces show FRET efficiencies captured over 90 s. ( G ) Schematic of SiMPull assay for FRET non-biotinylated FRET TELO4 construct binding to BG4 attached to the slide via FLAG antibody. ( H ) Histogram of FRET efficiency after adding non-biotinylated TELO4 FRET construct. ( I, J ) Dwell times for the high-FRET state of G2T and 8oxoG2 constructs in the presence and absence of BG4 from panels E and F. Colors indicate conformations for a range of FRET efficiencies: orange for partially unfolded GQ (marked with U) and blue for folded GQ (marked with F).

    Journal: Nucleic Acids Research

    Article Title: BG4 antibody can recognize telomeric G-quadruplexes harboring destabilizing base modifications and lesions

    doi: 10.1093/nar/gkad1209

    Figure Lengend Snippet: BG4 addition promotes GQ folding of telomeric constructs containing a destabilizing G substitution. Schematics for biotinylated FRET constructs TELO4 ( A ), G2T ( B ), or 8oxoG2 ( C ) tethered to neutravidin coated slides, followed by 150 nM BG4 addition and then buffer washes. Construct sequences shown in . Histograms of FRET efficiency frequencies observed TELO4 ( D ), G2T ( E ), and 8oxoG2 ( F ) before or after BG4 addition, and sequential buffer wash and SDS addition as indicated. Representative single-molecule traces show FRET efficiencies captured over 90 s. ( G ) Schematic of SiMPull assay for FRET non-biotinylated FRET TELO4 construct binding to BG4 attached to the slide via FLAG antibody. ( H ) Histogram of FRET efficiency after adding non-biotinylated TELO4 FRET construct. ( I, J ) Dwell times for the high-FRET state of G2T and 8oxoG2 constructs in the presence and absence of BG4 from panels E and F. Colors indicate conformations for a range of FRET efficiencies: orange for partially unfolded GQ (marked with U) and blue for folded GQ (marked with F).

    Article Snippet: Initially, the biotinylated mouse anti-FLAG monoclonal M2 antibody (F9291, Sigma) was diluted ∼500 times in T50 buffer (10 mM Tris–HCl, pH 7.5, 50 mM NaCl) and immobilized in the imaging chamber via biotin−NeutrAvidin interaction.

    Techniques: Construct, Binding Assay